PureAnalyt is a physics-faithful liquid chromatography simulator. Retention, efficiency, pressure and detection are modeled and anchored to dozens of real application notes. Built for chemists, technicians, educators and QC in pharma, food, environmental and biotech.
Every module of the chromatograph is modeled with real physics: configure your system, inject your sample, read the peaks.
Snyder LSS equation, Van Deemter, Darcy, the Hydrophobic Subtraction Model (HSM), and Henderson-Hasselbalch for pH-dependent retention.
Build it step by step: sample, degasser, pump (iso/binary/quaternary), autosampler, column oven, column and detector.
See tR, k', theoretical plates N, resolution (Rs), asymmetry and area. Hover any peak for full metrics. Optional MS spectrum.
LC-MS and LC-MS²: 3 ionization sources (ESI+/ESI-/APCI) × 3 modes (Full scan, SIM, MRM). LOD 1 ng → 1 pg depending on mode.
49 simulatable columns across 6 modes (RP, NP, HILIC, ligand-exchange, IEX, SEC). The equivalence advisor compares 62 commercial reference columns by similarity — dimensions, USP L-number and HSM coefficients (Snyder-Dolan Fs).
Real-time warnings: over-pressure, predicted co-elutions, silanol-driven tailing, HILIC issues, detector/column mismatch.
The physics models and databases cover the most common matrices in industrial and academic instrumental analysis.
Pick from a library of 208+ common analytes (drugs, pesticides, sugars, vitamins, cannabinoids, mycotoxins, proteins) or add your own with real physicochemical properties.
Configure every module: mobile phase, pump, autosampler, column oven, column and detector. Real-time diagnostics catch problems before you inject.
Chromatogram with integrated peaks, a full results table, optional MS spectrum. Export CSV. Iterate on the method and compare.
No. PureAnalyt runs entirely in your browser. Works on modern Chrome, Edge, Firefox and Safari. It sends no data to any server — the whole simulation is local.
Yes, the simulator is free to use. No signup, no card, no usage limits. A Pro version with advanced features (run comparison, reports, batch processing) is planned, but the core simulator will stay free.
Retention times, pressures, efficiencies and resolutions are modeled with validated equations (Snyder, Van Deemter, HSM Snyder-Dolan, Darcy, Henderson-Hasselbalch). The simulator does not replace a real instrument for regulatory validation, but produces results consistent with theory and published data.
Yes — it is purpose-built for it. The step-by-step modular structure, diagnostic warnings and tooltips make it an excellent tool for chromatography, analytical chemistry and QC courses.
In the current version, not permanently — the state resets on refresh. This is one of the first features of the Pro version in development. For now you can export your chromatograms as CSV.
Write to contacto@pureanalyt.com. All feedback is welcome — we are actively improving.